Scar

Part:BBa_K2355317

Designed by: Chrysillis Polhaus   Group: iGEM17_DTU-Denmark   (2017-10-26)


Double cut side containing NgoMIV and AgeI, with a frameshift and stop codon in between.

The cut side that allows insertions of RFC[25] parts. The cut side are NgoMIV and AgeI, between the cut sides there is a stop codon and a frameshift. If insertion of RFC[25] part is not succesful the succeeding part will not be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
    Illegal AgeI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12


[edit]
Categories
Parameters
None